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Mouse Cxcl13/Blc/Bca 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) <t>CXCL13</t> (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.
Cxcl13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) <t>CXCL13</t> (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.
Mouse Cxcl13 Blc Bca 1 Antibody Af470, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) <t>CXCL13</t> (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.
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(A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) <t>CXCL13</t> (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.
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(A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) CXCL13 (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.

Journal: PLOS One

Article Title: Aligning cellular and molecular components in age-dependent tertiary lymphoid tissues of kidney and liver

doi: 10.1371/journal.pone.0311193

Figure Lengend Snippet: (A) Hematoxylin and eosin (H&E) staining. Quantitative analysis of (B) TLT frequencies, (C) TLT numbers, and (D) relative TLT sizes in young and superaged mouse livers (n = 6 per group). Immunofluorescence analysis of (E) lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and smooth muscle actin (α-SMA); (F) CD3ε (a T-cell marker) and B220 (a B-cell marker); (G–I) CD21 (a follicular dendritic cell (FDC) marker) and Ki67; (J) B220 and Ki67; (K, L) CD45 and p75 neurotrophin receptor (p75NTR); (M) p75NTR and CD21; (N, O) Desmin (a hepatic stellate cell marker) and p75NTR; (P) CXCL13 (a homeostatic chemokine) and CD45; (Q) CCL19 (a homeostatic chemokine) and CD45; (R) Desmin and CXCL13; and (S) CD21 and CXCL13. (T) mRNA expression of Cd3e , Cd19 , Des ( Desmin ), and Ngfr ( p75ntr ) in young and superaged mouse livers (n = 6 per group). (U) Correlations between relative TLT sizes and mRNA levels of Cxcl13 and Ccl19 in young and superaged mouse livers (n = 6 per group). The mRNA expression levels are normalized to those of Gapdh and expressed relative to those of the young mouse. (V) Representative immunofluorescence of three distinct stages (I, II, and III) of TLTs. Immunofluorescence analysis of CD3εand B220; CD21 and Ki67 in each stage are serial sections. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal centers; Stage III TLTs include FDCs and germinal centers. (W) The relative numbers of stages I, II, and III TLTs and the proportions of stages I, II, and III TLTs in superaged mouse livers without injury (n = 6). In (C, D, T, W) data are shown as the median and interquartile range. Mann–Whitney test (C, D, T) was used to analyze the difference. Correlations in (U) are determined by Pearson’s correlation analysis. TLT number is shown as the number per area of section. * p ≤ 0.05 and ** p ≤ 0.01. Arrows indicate TLT localization. Scale bars: 50 µm. Abbreviations: CV, central vein; PT, p ortal tract.

Article Snippet: Sections were blocked with 5% serum appropriate for the secondary antibody for 1 h at room temperature, and then were incubated overnight at 4°C with primary antibodies as follows: anti-LYVE-1 (catalog no. ab14917; Abcam, Cambridge, UK), -α-SMA (catalog no. C6198; Sigma-Aldrich, Missouri, USA), -CD3ε (catalog no. 550275; BD PharMingen, San Jose, CA, USA), -B220 (catalog no. 557390; BD PharMingen), -CD21 (catalog no. ab75985; Abcam), -Ki67 (catalog no. 14-5698; eBioscience, San Diego, CA, USA, catalog no. ab15580; Abcam), -CD45 (catalog no. 14-0451; eBioscience), -p75NTR (catalog no. AF1157; R&D Systems, Minneapolis, MN, USA), -Desmin (catalog no. PA5-16705; Thermo Fisher Scientific, Waltham, MA, USA), -CXCL13 (catalog no. AF470; R&D Systems), -CCL19 (catalog no. AF880; R&D Systems), -CD11c (catalog no. 550283; BD PharMingen), -p21 (catalog no. 188224; Abcam), -CD90.2 (catalog no. 553011; BD PharMingen), and -cytokeratin 19 (catalog no. ab52625; Abcam) antibodies.

Techniques: Staining, Immunofluorescence, Marker, Expressing, MANN-WHITNEY

(A–K) Histological analyses of HCV patients’ liver samples. (A, B) Hematoxylin and eosin (H&E) and (C) silver stain. Immunofluorescence analysis of (D) CD3ε (a T-cell marker) and CD20 (a B-cell marker); (E) Ki67 and CD23 (a follicular dendritic cell marker); (G) Desmin (a satellite cell marker); (H) α-SMA and (I) CXCL13 (a homeostatic chemokine) and CD45. (F) Immunohistochemical analysis of CD21 (a follicular dendritic cell marker). (J) In situ hybridization images of TNFSF8 with immunofluorescence co-staining for CD3ε (K) Representative immunofluorescence analysis of two distinct stages (I and II) of TLTs. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal center. Immunofluorescence analysis of CD3ε and CD20; Ki67 and CD23 in each stage are serial sections. (L–N) Quantification of hepatic TLTs in young and aged patients with HCV infection (n = 22 and 25, respectively). (L) TLT frequencies in young and aged patients are 72.7% and 100%, respectively. (M) The relative numbers of TLTs per portal tract number. The medians of those in young and aged patients are 0.125 and 0.138, respectively. (N) The proportion of patients in each TLT stages. The proportion of patients with stage I TLTs in young and aged patients are 45% and 48%, respectively, while that of patients with stage II TLTs are 27% and 52%, respectively. (O) Percentage of patients in each fibrosis stage according to the new Inuyama classification stratified by hepatic TLT presence (no TLT: n = 6, with TLTs: n = 41). Arrows indicate TLT localization. Scale bars: in (A) 200 µm; (B–K) 50 µm. Abbreviations: CV, central vein; PT, portal tract.

Journal: PLOS One

Article Title: Aligning cellular and molecular components in age-dependent tertiary lymphoid tissues of kidney and liver

doi: 10.1371/journal.pone.0311193

Figure Lengend Snippet: (A–K) Histological analyses of HCV patients’ liver samples. (A, B) Hematoxylin and eosin (H&E) and (C) silver stain. Immunofluorescence analysis of (D) CD3ε (a T-cell marker) and CD20 (a B-cell marker); (E) Ki67 and CD23 (a follicular dendritic cell marker); (G) Desmin (a satellite cell marker); (H) α-SMA and (I) CXCL13 (a homeostatic chemokine) and CD45. (F) Immunohistochemical analysis of CD21 (a follicular dendritic cell marker). (J) In situ hybridization images of TNFSF8 with immunofluorescence co-staining for CD3ε (K) Representative immunofluorescence analysis of two distinct stages (I and II) of TLTs. Stage I TLTs are the aggregation of lymphocytes with the sign of proliferation, and do not include FDCs or germinal centers; Stage II TLTs include FDCs, but not germinal center. Immunofluorescence analysis of CD3ε and CD20; Ki67 and CD23 in each stage are serial sections. (L–N) Quantification of hepatic TLTs in young and aged patients with HCV infection (n = 22 and 25, respectively). (L) TLT frequencies in young and aged patients are 72.7% and 100%, respectively. (M) The relative numbers of TLTs per portal tract number. The medians of those in young and aged patients are 0.125 and 0.138, respectively. (N) The proportion of patients in each TLT stages. The proportion of patients with stage I TLTs in young and aged patients are 45% and 48%, respectively, while that of patients with stage II TLTs are 27% and 52%, respectively. (O) Percentage of patients in each fibrosis stage according to the new Inuyama classification stratified by hepatic TLT presence (no TLT: n = 6, with TLTs: n = 41). Arrows indicate TLT localization. Scale bars: in (A) 200 µm; (B–K) 50 µm. Abbreviations: CV, central vein; PT, portal tract.

Article Snippet: Sections were blocked with 5% serum appropriate for the secondary antibody for 1 h at room temperature, and then were incubated overnight at 4°C with primary antibodies as follows: anti-LYVE-1 (catalog no. ab14917; Abcam, Cambridge, UK), -α-SMA (catalog no. C6198; Sigma-Aldrich, Missouri, USA), -CD3ε (catalog no. 550275; BD PharMingen, San Jose, CA, USA), -B220 (catalog no. 557390; BD PharMingen), -CD21 (catalog no. ab75985; Abcam), -Ki67 (catalog no. 14-5698; eBioscience, San Diego, CA, USA, catalog no. ab15580; Abcam), -CD45 (catalog no. 14-0451; eBioscience), -p75NTR (catalog no. AF1157; R&D Systems, Minneapolis, MN, USA), -Desmin (catalog no. PA5-16705; Thermo Fisher Scientific, Waltham, MA, USA), -CXCL13 (catalog no. AF470; R&D Systems), -CCL19 (catalog no. AF880; R&D Systems), -CD11c (catalog no. 550283; BD PharMingen), -p21 (catalog no. 188224; Abcam), -CD90.2 (catalog no. 553011; BD PharMingen), and -cytokeratin 19 (catalog no. ab52625; Abcam) antibodies.

Techniques: Silver Staining, Immunofluorescence, Marker, Immunohistochemical staining, In Situ Hybridization, Staining, Infection